Fig. 2.

Identification of C. glutamicum strains with different signal peptides for cutinase secretion based on extracellular cutinase activities. a Cutinase activities obtained from MPP cultivations using single C. glutamicum colonies with defined signal peptide insert for inoculation. Colonies were plated from a frozen cryostock of the corresponding expression strain. Error bars indicate standard deviation from eight replicates. b Likewise, but for obtaining agar colonies a mixture of the frozen cryostocks was plated yielding single colonies with undefined signal peptide insert for cutinase secretion. From these, 48 colonies were randomly picked as inoculation material for 48 MPP cultivations. Error bars indicate standard deviations from three cutinase measurements