Table 1.
Comparison of extracellular cutinase activities depending on Sec signal peptide for C. glutamicum (this study) or B. subtilis (as reported by Brockmeier et al. [16])
| Signal peptide | C. glutamicum | B. subtilis | ||
|---|---|---|---|---|
| Activity [U/mL] |
Relative activity [%] |
Activity [U/mL] |
Relative activity [%] |
|
| Epr | 13.1 ± 0.8 | 100.0 ± 5.8 | 4.7 | 100.0 |
| YwfM | 11.2 ± 1.4 | 85.5 ± 10.4 | 0.4 | 7.7 |
| Bpr | 11.1 ± 1.0 | 84.8 ± 7.9 | 3.0 | 63.6 |
| Vpr | 10.5 ± 0.1 | 80.2 ± 0.6 | 2.2 | 47.1 |
| YkvV | 9.4 ± 1.9 | 72.0 ± 14.1 | 1.0 | 21.8 |
| YurI | 9.4 ± 0.6 | 71.9 ± 4.9 | 1.9 | 41.5 |
| YxiT | 9.4 ± 0.3 | 71.4 ± 2.0 | 0.9 | 19.3 |
| LipB | 9.2 ± 2.1 | 70.2 ± 15.9 | 1.6 | 34.7 |
| YurI | 8.6 ± 0.6 | 65.6 ± 4.4 | 1.9 | 41.5 |
| YckD | 8.5 ± 2.3 | 64.9 ± 17.7 | 2.8 | 59.7 |
| YfjS | 8.0 ± 0.3 | 60.8 ± 2.2 | 0.5 | 10.5 |
| Epr | 7.8 ± 1.6 | 59.8 ± 12.1 | 4.7 | 100.0 |
| YxaK | 7.8 ± 1.2 | 59.5 ± 9.1 | 0.0 | 0.0 |
| Csn | 7.5 ± 2.0 | 57.4 ± 15.5 | 3.4 | 71.7 |
| YxaK | 8.0 ± 2.3 | 60.9 ± 17.5 | 0.0 | 0.0 |
| YndA | 7.2 ± 3.5 | 55.0 ± 27.0 | 0.5 | 11.1 |
| YxaK | 7.1 ± 0.3 | 54.0 ± 2.4 | 0.0 | 0.0 |
| YbdN | 6.9 ± 1.2 | 52.4 ± 9.1 | 2.5 | 53.7 |
| YobB | 6.7 ± 0.6 | 50.9 ± 4.8 | 2.5 | 53.3 |
| YndA | 6.2 ± 3.6 | 47.2 ± 27.3 | 0.5 | 11.1 |
| YkvV | 6.1 ± 0.6 | 46.5 ± 4.6 | 1.0 | 21.8 |
| YurI | 5.6 ± 0.9 | 42.7 ± 6.5 | 1.9 | 41.5 |
| YwtD | 5.9 ± 1.1 | 45.2 ± 8.6 | 0.3 | 5.4 |
| YddT | 5.7 ± 0.5 | 43.6 ± 4.0 | 2.4 | 51.6 |
| YbbE | 5.6 ± 0.0 | 42.7 ± 0.1 | 2.1 | 44.8 |
| Mdr | 5.5 ± 0.1 | 42.1 ± 0.5 | 0.4 | 8.8 |
| YobV | 5.0 ± 0.2 | 38.5 ± 1.5 | 0.0 | 0.0 |
| YvpA | 5.0 ± 0.5 | 38.2 ± 3.9 | 1.9 | 40.0 |
| YwmC | 4.6 ± 0.2 | 35.2 ± 1.2 | 1.2 | 25.1 |
| YbbE | 4.5 ± 0.5 | 34.4 ± 4.0 | 2.1 | 44.8 |
| YwmD | 4.5 ± 0.4 | 34.4 ± 2.8 | 0.3 | 5.6 |
| YckD | 4.4 ± 0.1 | 33.9 ± 0.7 | 2.8 | 59.7 |
| YvgO | 3.6 ± 0.3 | 27.5 ± 2.4 | 0.0 | 0.0 |
| TasA | 3.4 ± 3.0 | 26.1 ± 22.5 | 0.3 | 6.2 |
| YqxI | 3.2 ± 0.2 | 24.7 ± 1.4 | 1.4 | 30.2 |
| YndA | 3.2 ± 0.6 | 24.3 ± 4.7 | 0.5 | 11.1 |
| YogH | 2.7 ± 1.7 | 20.4 ± 12.9 | 0.0 | 0.0 |
| YfkN | 2.1 ± 0.0 | 16.3 ± 0.2 | 0.2 | 4.5 |
| LytC | 2.1 ± 1.3 | 16.2 ± 9.6 | 0.9 | 19.3 |
| WprA | 2.1 ± 1.7 | 15.8 ± 13.0 | 0.1 | 2.6 |
| YobV | 2.0 ± 1.3 | 15.2 ± 9.8 | 0.0 | 0.0 |
| YbdN | 1.9 ± 1.1 | 14.9 ± 8.6 | 2.5 | 53.7 |
| Pel | 1.9 ± 0.5 | 14.5 ± 3.7 | 2.7 | 57.2 |
| YycP | 1.9 ± 2.1 | 14.2 ± 16.3 | 0.0 | 0.0 |
| YogH | 1.7 ± 0.3 | 12.9 ± 2.3 | 0.0 | 0.0 |
| YvpB | 1.6 ± 1.7 | 12.2 ± 13.2 | 0.0 | 0.0 |
| AmyE | 1.5 ± 0.2 | 11.5 ± 1.8 | 0.7 | 14.3 |
| YogH | 1.3 ± 0.1 | 10.1 ± 0.5 | 0.0 | 0.0 |
| TasA | 1.2 ± 0.2 | 9.5 ± 1.5 | 0.3 | 6.2 |
| AmyE | 1.0 ± 0.1 | 7.8 ± 0.8 | 0.7 | 14.3 |
| AmyE | 0.9 ± 0.2 | 7.2 ± 1.8 | 0.7 | 14.3 |
| YbbE | 0.9 ± 0.5 | 6.9 ± 4.1 | 2.1 | 44.8 |
| YogH | 0.9 ± 0.2 | 6.6 ± 1.4 | 0.0 | 0.0 |
| YycP | 0.8 ± 0.8 | 6.3 ± 6.1 | 0.0 | 0.0 |
| DacF | 0.8 ± 0.2 | 5.8 ± 1.2 | 0.1 | 3.0 |
| RpmG | 0.7 ± 0.6 | 5.4 ± 4.6 | 0.1 | 3.0 |
| YycP | 0.7 ± 0.7 | 5.4 ± 5.6 | 0.0 | 0.0 |
| YwgB | 0.7 ± 0.2 | 5.3 ± 1.8 | 0.0 | 0.0 |
| YwtD | 0.5 ± 0.3 | 3.7 ± 2.4 | 0.3 | 5.4 |
| YdbK | 0.4 ± 0.1 | 3.0 ± 0.4 | 0.2 | 4.7 |
| YwtF | 0.3 ± 0.0 | 2.5 ± 0.3 | 0.0 | 0.0 |
| YwgB | 0.3 ± 0.1 | 2.5 ± 0.9 | 0.0 | 0.0 |
| YqzC | 0.3 ± 0.1 | 2.0 ± 0.5 | 0.0 | 0.0 |
| YvpB | 0.0 ± 0.0 | 0.3 ± 0.1 | 0.0 | 0.0 |
Signal peptides are sorted by their corresponding extracellular cutinase activity for C. glutamicum, multiple entries are found for Sec SPs that have been identified more than once. For these, the same cutinase activities reported by Brockmeier et al. have been assigned. Cutinase activities are given as absolute and relative values with respect to the maximal activity of the corresponding data series. Cutinase activity errors for C. glutamicum represent standard deviations from two analytical replicates. Cutinase activity errors for B. subtilis are 25%, as reported [16]
Three italics marked SPs (Bpr, Pel, YwfM) with their corresponding extracellular cutinase activities in both secretion hosts are discussed in the text, cf. also Fig. 4