Fig. 3.

Characterization of purified IFN-α/Fc fusion proteins. a Comparison of purified IFN-α/Fc fusion proteins using SDS-PAGE under reduced and non-reduced conditions. b Western blot analysis of the purified IFN-α/Fc fusion proteins. The indicated samples were separated via reduced SDS-PAGE and identified through Western blot analysis with goat anti-human IgG-HRP conjugate or mouse anti-human IFN-α monoclonal antibodies, followed by rabbit anti-mouse IgG-HRP conjugate. c PAS staining of purified IFN-α/Fc fusion proteins via SDS-PAGE under reduced conditions. WT: IFN-α/Fc-WT, MD: IFN-α/Fc-MD, SC: IFN-α/Fc-SC, M: prestained protein marker