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. 2016 Dec 7;3:16026. doi: 10.1038/mto.2016.26

Figure 1.

Figure 1

Schematic of chimeric antigen receptor (CAR) structures containing the BCL or CD5 variable lymphocyte receptor (VLR) is shown along with the structure of the mature VLR gene. (a) A depiction of the mature VLR gene which is assembled from a series of leucine rich repeat (LRR) cassettes spliced from the germ line cell to the mature B-like cell in a combinatorial fashion. SP, signal peptide; LRRNT, N-terminal leucine-rich repeat; LRRV, variable leucine-rich repeats; LRRVe, end leucine-rich repeat; CP, connecting peptide; LRRCT, C-terminal leucine-rich repeat; HP - hydrophobic peptide. (b) The structure of the BCL-CAR transgene includes a 5′ long terminal repeat (LTR), human ubiquitin C promoter (hUBC), an interleukin-2 signal peptide (IL-2 SP), the BCL VLR, a myc epitope tag, the CD28 region, the CD3-ζ intracellular domain and a 3′ LTR. (c) The structure of the CD5-VLR-CAR transgene.