Fig. 1.

Association of downregulated in adenoma (DRA) and DRA-ETKFminus with lipid rafts in stably transfected HEK cells. HEK/EGFP-DRA (A) and HEK/EGFP-DRA-ETKFminus cells (B) were lysed at 4°C using 1% Triton X-100. The lysates were subjected to sucrose density centrifugation. As a raft marker flotillin was detected in the low-density fractions 3-6 and as a nonraft marker rab5 was detected in the high-density fractions. Both EGFP-DRA and EGFP-DRA-ETKFminus were consistently detected in both low-density (lipid raft) as well as high-density (nonraft) fractions, suggesting that DRA is partially localized within lipid rafts independently of its interaction with PDZ adaptor proteins. Representative data of 5 experiments. EGFP, enhanced green fluorescent protein.