Fig. 2.
S1045Y-cystic fibrosis transmembrane conductance regulator (CFTR) exhibits reduced function compared with wild-type (WT)-CFTR. A: DNA chromatogram to depict generation of Ser-to-Tyr mutation by conversion of cytosine to adenine nucleotide at position 3134. B: Western blot showing that S1045Y-CFTR has similar band C and B configurations as those of WT-CFTR. C: iodide efflux measurements in HEK 293 cells showing that S1045Y-CFTR exhibits ∼50% channel activity of normal CFTR. D: left, representative CFTR-mediated short-circuit currents (Isc) in CFBEo− cells. Right, data quantification from experiments as shown on left. Data were normalized to S1045Y-CFTR-mediated Isc from n = 3 independent experiments.