Fig. 10.

Lp-PLA₂ exacerbated the AAPH-treated BHCL decrease in transendothelial cell electrical resistance (increase monolayer permeability). BPAECs (30,000 cells) were plated and grown to a confluent monolayer and exposed to BHCL in HBSS for 1 h, and permeability was determined by ECIS array experiments run with the 8W10E+ polyethylene terephthalate sterile disposable electrode array, with or without a nontoxic dose of AAPH with or without 2,000 ng/ml Lp-PLA₂. Thrombin was used as a positive control for an increase in monolayer permeability (green line). Data from each trial were averaged at specific time points and then plotted vs. time with ± error bars reflecting standard errors from the trials. *P < 0.05.