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EPR reveals the presence of free ascorbyl radicals generated by HBSS in a cell-free system. EPR spectra shown of a cell-free in vitro system containing HBSS were recorded 10 and 20 s after addition of 25 μM ascorbate (A), then metal chelator, DTPA (100 μM; B). C shows the addition of the free radical generator AAPH (5 mM) to 25 μM ascorbate, then addition of 100 μM DTPA (D), followed by the addition of 100 μM BHT (E).
