Fig. 4.

BHCL in the presence of AAPH produced multiple oxidation products as shown by LC-MS/MS. LC-MS/MS was performed on the reduced form on BHCL (A) and BHCL + AAPH (B). A indicates 2 CL clusters at m/z 1,469 (minor), 1,447 (major). B indicates multiple CL clusters, the largest at m/z 1,447, 1,469, 1,479, 1,495 and 1,511, 1,527, 1,543, and 1,559. There was a decrease in the major m/z 1,447 CL species and increases in species at the other m/z displayed in gray representing various oxidized forms of CL with or without adducts. Each of these oxidized forms of CL having an additional oxygen molecule added was determined by the m/z increase of 16.