Table 1.

Promoter analysis on genes that may regulate BDNF secretion by ASM cells

Gene	CREB	NFAT
BDNF	−88 to −80	−284 to −277
		−366 to −359
CAPS2	−7782 to −7775	−957 to −950
		−1348 to −1341
		−1509 to −1502
SNAP25	−3107 to −3100	−569 to −562
		−3793 to −3786
		−3802 to −3795
SYT4	−1150 to −1143	−765 to −758
	−5629 to −5622	−1581 to −1574
	−5776 to −5769

Approximately 10–12-kb region, upstream of start codon (ATG), was analyzed for putative cAMP response element binding protein (CREB) or nuclear factor of activated T cells (NFAT) binding sites, based on published consensus sequences (^5′TGACGTCA^3′ for CREB and ^5′AGGAAATT^3′ or ^5′AGGAAACA^3′ for NFAT).

BDNF, brain-derived neurotrophic factor; ASM, airway smooth muscle. Binding site location is presented as distance from the start codon.