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. 2016 Dec 7;3:16032. doi: 10.1038/mto.2016.32

Figure 2.

Figure 2

MYXV induces apoptosis in MOPC-315 cells (a) The indicated cell lines were infected with MYXV at an MOI = 5. At various times postinfection, cells were harvested and the numbers of new infectious progeny were determined using foci forming assays. Data is displayed as the fold increase in progeny over that observed at 6 hours postinfection. MOPC-315 cells were mock-infected or infected with MYXV at an MOI = 10. (b) Six hours postinfection, cells were stained with annexinV (AnV) and propidium iodide (PI). (c) At the indicated times postinfection, cells were harvested and the cleavage of the indicated apoptotic proteins was analyzed using western blot. (d) MOPC-315 cells or U266 cells were mock-infected or infected with MYXV at an MOI = 10 in either the presence of absence of cytarabine (AraC). Cellular viability was then determined 24 hours after infection using MTT assay. Significance was determined using student’s t-test (***P < 0.0001). MM, multiple myeloma; MYXV, myxoma virus; MOI, multiplicity of infection.