Figure 4.

MYXV injection does not inhibit the functionality of the BM niche. Balb/C mice were given three injections of either saline (n = 10) or 1 × 10⁸ FFU of vMYX-GFP (n = 10) over 5 days. Twenty-four hours after the final infection, animals were killed and the (a) numbers of cells in the BM of each mouse was determined using a hemocytometer while the (b) infection of these cells was determined using flowcytometry. Significance was determined using student’s t-test. Balb/C mice were lethally irradiated (700 cGy) and then injected with either saline (n = 2) or 5 × 10⁶ syngeneic BM cells form a nonirradiated Balb/C donor. Transplanted animals were then separated into two groups and given three injections of either saline (n = 10) or 1 × 10⁸ FFU of MYXV (n = 12) over 5 days. (c) Body weight was monitored twice weekly and (d) animals were killed when they dropped below 75% of their starting weight. Significance was determined using student’s t-test at each time point. BM, bone marrow; GFP, green fluorescent protein; FFU, foci forming units; MYXV, myxoma virus.