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. 2016 Dec 7;3:16071. doi: 10.1038/mtm.2016.71

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

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Purification of baculovirus supernatants with heparin affinity chromatography. Baculovirus sample was purified using a POROS heparin column with optimal conditions for sample loading and washing as described. Virus was eluted with 1.5 mol/l NaCl and diluted with phosphate-buffered saline (PBS). Baculovirus infectivity was estimated by infection of HT1080 cells with the diluted fractions of baculovirus. The line with dark diamonds shows the total infectious units (IU) of baculovirus in billions in each fraction.