Figure 1.

scAAV9-CBA-GFP, scAAV9-GFA-GFP, and scAAV9-Syn-GFP efficiently transduce the murine central nervous system after a single intravenous injection of (2 × 10¹³ vg/kg). (a) Vector maps of the scAAV expression cassettes used in the study as well as representative images of the GFP signal across an entire sagittal section of the brain after a single intravenous injection of scAAV9-CBA-GFP, scAAV9-GFA-GFP or scAAV9-Syn-GFP (left panels, scale bar = 1,000 μm). The right panels show the overall fluorescent signal within the spinal cord after similar injections (dh and vh: dorsal and ventral horns; scale bar = 500 μm). (b) A substantial number of GFP-positive cells could be detected throughout the entire cerebral tissue, as shown in the cortex, striatum, cerebellum or hippocampus (CA, Cornus Ammonis; DG, Dentate Gyrus; scale bar = 100 μm).