Figure 5.

scAAV9-Syn-GFP allows expression of a specific transgene in various neuronal subtypes. Peripheral infusion of scAAV9-Syn-GFP led to efficient transduction of both excitatory (a) as well as inhibitory (b) neurons (respectively identified by the CamKII and GAD67 markers). TH-positive neurons in the substantia nigra rarely expressed GFP (c). Few transduced Purkinje cells could also be observed in the cerebellum (d), their density being much lower as compared with what has been previously reported using a CBA promoter.²¹ Importantly, ChAT-positive motor neurons expressing GFP were also observed in the spinal cord (e). CamKII, Ca2+ /calmodulin-dependent protein kinase II; ChAT, choline acetyltransferase; GAD67, glutamic acid decarboxylase; TH, tyrosine hydroxylase; scale bar = 50 μm.