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. 2016 Dec 7;3:16029. doi: 10.1038/mto.2016.29

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

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Use of a modified transwell assay to assess a TNT-mediated bystander effect following oncolytic viral infection. (a) Schematic drawing of the modified transwell assay/Boyden chamber used to assess TNT-mediated transfer. (b) Representative fluorescence micrographs of TUNEL staining of apoptotic MSTO-211H mesothelioma cells in the bottom well of the transwell assay. Green fluorescence shows virus-encoded eGFP expression. Red fluorescence shows TUNEL-positive nuclei. Blue fluorescence indicates expression of Caspase-3. eGFP, enhanced green fluorescence protein; TNT, tunneling nanotube; TUNEL, terminal deoxynucleotidyl transferase nick-end labeling.