Animals with CIBP, induced by injecting and confining tumor cells to one femur, treated with vehicle (PBS; light gray bars), developed mechanical hypersensitivity of the plantar surface of the hindpaw. This hypersensitivity of the skin increased with time and was present in the ipsilateral (A) but not contralateral plantar surface of the hindpaw (B). Sustained treatment with anti-P2X3 (30 mg/kg, i.p.; dark gray bars) significantly relieved mechanical hypersensitivity of the skin in sarcoma-injected mice at days 28, and 35 when compared to sarcoma + vehicle-treated animals. Anti-NGF treatment (10 mg/kg, i.p.; black bars) significantly reversed mechanical hypersensitivity of the skin at days 21, 28, and 35 when compared to vehicle-treated bone cancer bearing mice. Dashed line represents the baseline (naïve, pre-surgery) values. Error bars represent S.E.M. * P≤0.05 vs. vehicle, ** P≤0.01 vs vehicle.