TABLE 2 .
The 15 most eDNA-deficient mutants from M. avium subsp. hominissuis A5 Mmt7 library screen
| Name | A5 gene | Encoded protein | eDNA/ODa |
|---|---|---|---|
| 11e7 | MAVA5_03380 | FtsK/SpoIIIE | 6,177 |
| 2e3 | MAVA5_19115 | Hypothetical (WXG100 domain) | 6,432 |
| 29e7 | MAVA5_10575 | ABC transporter permease | 6,619 |
| 42c3 | MAVA5_13685 | Hypothetical (Major facilitator superfamily domain) | 6,654 |
| 39e5 | MAVA5_20295 | SAM-dependent methyltransferase | 6,798 |
| 40c10 | MAVA5_19945 | Carbonic anhydrase | 6,941 |
| 24d3 | MAVA5_13900 | Hypothetical protein (dehydrogenase domain) | 7,105 |
| 41c1 | MAVA5_05585 | Transcription-repair coupling factor | 7,184 |
| 36d7 | MAVA5_05310 | (2Fe−2S)-binding protein | 7,469 |
| 40h2 | MAVA5_10220 | Hypothetical protein (MaoC dehydrogenase domain) | 7,581 |
| 1e5 | MAVA5_10615 | SAM-dependent methyltransferase | 7,871 |
| 36f9 | MAVA5_13185 | Oxidoreductase | 7,932 |
| 35c4 | MAVA5_01245 | Hypothetical protein (19 kDa mycobacterial antigen domain) | 7,960 |
| 39d10 | MAVA5_13215 | Cytochrome P450 | 8,000 |
| 14g4 | MAVA5_07205 | Hypothetical protein (Domain of unknown function) | 8,077 |
a
The eDNA/optical density (OD) value for each sample was calculated by normalizing the fluorescence reading for day 7 RFU bound to PI to the starting OD of the respective sample.