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The LCD supernatant is toxic. The LCD-locked mutant (luxOD61E ΔaphA ΔhapR ΔvpsL) (red) and the HCD-locked mutant (ΔluxO ΔaphA ΔhapR ΔvpsL) (blue) were measured at 72 h of growth in HCD SCM (solid bars) or LCD SCM (checkered bars). The blue dotted line indicates the initial cell viability of each strain at 0 h. The limit of detection at 72 h is 2 cells/ml of culture. The values shown are averages of at least three replicates. Error bars denote the SEM.
