FIG 6 .
N. gonorrhoeae ΔpilT and pilTL201C cells fail to induce transcription of EGFR ligands and shedding of HB-EGF. (A) mRNA levels of hbegf (left) and areg (right) in N. gonorrhoeae wt-, ΔpilT- and pilTL201C-infected cultures were determined by real-time PCR; the values were normalized to the value for the GAPDH internal control and expressed relative to the value for the uninfected control (UI). Values are the average results from 3 independent experiments. *, P < 0.05, and **, P < 0.01, Student’s two-tailed unpaired t test. (B) Results of Western dot blot of supernatants from infected cultures for soluble HB-EGF. Signals were normalized to the value for uninfected-cell supernatant. Values are the average results from 3 independent experiments. *, P < 0.05, Student’s two-tailed unpaired t test.