Skip to main content
. Author manuscript; available in PMC: 2016 Dec 7.
Published in final edited form as: Sci Immunol. 2016 Jul 14;1(1):aaf8759. doi: 10.1126/sciimmunol.aaf8759

Fig. 1. Kinetics of T cell alloresponses after transplantation of allogeneic skin grafts.

Fig. 1

(A) C57Bl/6 (B6, H-2b) mice received a skin graft from a fully allogeneic BALB/c (H-2d) or a syngeneic B6 mouse. At different time points after transplantation, recipient LN T cells were isolated and cultured in vitro for 48 hours with donor irradiated spleen cells. (B) B6 mice were transplanted with a skin patch from a BALB/c mouse. Skin allografts were removed at different time points after transplantation. Recipient LN T cells were isolated at day 10 after transplantation and cultured for 48 hours with medium or with either allogeneic (BALB/c) or syngeneic (B6) spleen cells (APCs). In (A) and (B), the frequencies of IFN-γ–producing cells were measured by ELISPOT. The results are expressed as numbers of IFN-γ spots per million T cells ± SD (triplicate wells) isolated from mouse LNs collected and pooled from three to five mice. The results are representative of three separate experiments. The P values obtained with analysis of variance (ANOVA) comparing T cell responses after syngeneic versus allogeneic graft (A) or APCs (B) were P = 0.0005 and P = 0.0001.