Table 1. Frequencies of donor leukocytes and recipient cells cross-dressed with donor after skin transplantation.

Draining (ipsilateral) and nondraining (contralateral) LN cells (axillary and brachial LNs) and the spleen and thymus from naïve B6 and BALB/c mice as well as B6 mice recipient of a BALB/c skin allograft were collected at different time points after transplantation. The cells were stained with anti-MHC class I K^b antibodies and anti-MHC class I K^d bound to FITC and allophycocyanin, respectively. The presence of recipient (K^b+) and donor (K^d+) cells was assessed using flow imaging with low-speed fluidics at ×40 magnification. The frequencies are expressed as the number of donor cells or recipient cells cross-dressed with allogeneic MHC molecules per million cells collected for each lymphoid organ. The results are representative of four to six mice tested individually. nd, not determined.

Days after skin grafting	Draining LNs	Nondraining LNs	Spleen	Thymus
Number of donor leukocytes (per million cells)
0.5	0	0	0	0
1.5	0	0	0	0
4	0	0	0	0
7	0	0	0	0
15	0	0	0	0
Number of cross-dressed cells (per million cells)
0.5	377 ± 141	270 ± 46	150 ± 29	nd
1.5	760 ± 74	607 ± 18	463 ± 48	330
4	1107 ± 207	870 ± 40	587 ± 70	430
7	1653 ± 359	710 ± 121	433 ± 15	200
15	367 ± 367	220 ± 82	227 ± 64	nd