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. 2016 Dec 7;12(12):e1006050. doi: 10.1371/journal.ppat.1006050

Fig 8. Type-I IFN induces IL-27 expression during MCMV infection.

Fig 8

(A) Heat map of IL-27 p28 mRNA expression by WT, IFNβ-/- and IFNαR-/- macrophages following stimulation with WT MCMV (C3X), replication-deficient (ΔIE3) MCMV or poly(I:C). (B) Expression of IL-27 p28 by splenic leukocytes was assessed d2 pi in Cre- mice treated/not with 2 mg anti-IFNαR-1. Mean + SEM of 8 mice/group is shown and data is representative of 2 separate experiments. (C) WT (C57BL/6) mice were infected with MCMV treated with anti-IL-27, Isotype or virus alone. At d6 pi mice were treated /not with anti-IL-10R and at d14 pi spleen CD4+/IFNγ+ (D) responses were quantified and expressed as mean ± SEM of 4–15 mice/group. (E) MCMV genomes in saliva were quantified by qPCR. Data shown as mean ± SEM from 4–15 mice/ group.