Fig 4. IFNAR blockade increases MCMV lytic infection in LN.

C57BL/6 mice were given IFNAR blocking antibody (αIFNAR) or not (control), then MCMV-GR i.f. (10⁶ p.f.u.). PLN taken 1 day later were stained for viral GFP, MCMV IE1 antigen, and LYVE-1 (lymphatic endothelium). (a) shows examples of staining. (b) shows quantitation, counting IE⁺ cells for 7 fields of view in PLN sections from each of 3 mice per group. Circles show individual means, bars show group means. αIFNAR significantly increased IE⁺ cell numbers (Student’s two tailed unpaired t-test with Welch’s Correction; ***, P<0.001).