Table 2.
A list of plasmid constructs used in this study.
| Plasmid Name | Content | Plasmid Construction | Donor Vecotr #1/att-PCRP-att | Donor Vector #2 | Donor Vector #3 | Donor Vector #4 |
|---|---|---|---|---|---|---|
| pWRPN01 | {pENTR L1-odr-1 promoter-L5r} | BP reaction: att-odr-1 promoter-att + pDONR P1-P5r | attB1-odr-1 promoter-attB5r | pDONR P1-P5r | ||
| pWRPN02 | {pDEST R1-chloramphenicol-ccdB-R2/pPD95.75} | pPD95.75 was cut with AgeI and EcoRI to remove GFP, then Gateway cassette RfA was inserted; | ||||
| pWRPN03 | {pENTR L1-ttx-3 element-L4} | BP reaction: att-ttx-3 element-att + pDONR P1-P4 | attB1-ttx- 3 element-attB4 | pDONR P1-P4 | ||
| pWRPN04 | {pENTR L5-DsRedT3-L2} | BP reaction | ||||
| pWRPN05 | {pENTR L1-lin-44 promoter-L5} | BP reaction | ||||
| pWRPN06 | {pENTR L5-NLS-G-GECO1.1-T2A-NLS-DsRed2-L2} | BP reaction: att-NLS-G-GECO1.1-T2A-NLS-DsRed2-att + pDONR P5-P2 | attB5-NLS-G-GECO1.1-T2A-NLS-DsRed2-attB2 | pDONR P5-P2 | ||
| pWRPN07 | {pExp B1-lin-44 promoter-B5-DsRed.T3-B2/pP95.75} | LR reaction: pWRPN05 + pWRPN04 + pWRPN02 | pWRPN05 | pWRPN04 | pWRPN02 | |
| pWRPN08 | {pExp odr-1 promoter-NLS-G-GECO1.1-T2A-NLS-DsRed2/pP95.75} | LR reaction: pWRPN01 + pWRPN06 + pWRPN02 | pWRPN01 | pWRPN06 | pWRPN02 | |
| pWRPN09 | {pEXP ttx-3 element-G-GECO1.1-T2A-DsRed2/pPD95.75} | LR reaction: pWRPN03 + pENTR L4-G-GECO1.1-L3 + pENTR L3-DsRed2-L2 + pWRPN02 | pWRPN03 | pENTR L4-G-GECO-L3 | pENTR L3-DsRed2-L2 | pWRPN02 |
| pWRPN10 | {L3613 rgef-1 promoter-NLS-G-GECO1.1-T2A-NLS-DsRed2} | |||||
| pWRPN11 | {L3613 tax-4 promoter-mNeptune} | |||||
| pWRPN12 | {L3613 glr-1 promoter-mNeptune} |