Table 3.

A list of genotyping performed in this study.

DOI: http://dx.doi.org/10.7554/eLife.19021.018

Mutation to Genotype	Forward Primer 5' --> 3'	Reverse Primer 5' --> 3'	Sequencing Primer	PCR Conditions*	Wild-type	Mutant
gcy-8 (oy44)	(WRO245) gcctaccaaattatttcaaacatc	(WRO246) TTGATAATTAAAATGCAAGACGAAC	N/A	Phusion Hot Start II; 1. 98°C, 1:00; 2. 98°C, 0:15; 3. 58°C, 0:30; 4. 72°C, 1:45; 5. Go to 2, 34x; 6. 72°C, 10:00; 7. 4°C	2225 bp band	750 bp < band < 1 kb
gcy-18 (nj38)	(WRO247) GAATAGAATGAGACGAATGAAATTTG	(WRO248) TGTTACCTACCAAGTGCCTAACTTAC	N/A	Phusion Hot Start II; 1. 98°C, 1:00; 2. 98°C, 0:15; 3. 58°C, 0:30; 4. 72°C, 1:45; 5. Go to 2, 34x; 6. 72°C, 10:00; 7. 4°C	1459 bp band	~500 bp band
gcy-23 (nj37)	(WRO252) CATCTACGGCTACATCCATCTC	(WRO253) TCCATCATACGCATCATCTG	N/A	Phusion Hot Start II; 1. 98°C, 1:00; 2. 98°C, 0:15; 3. 65°C, 0:30; 4. 72°C, 1:45; 5. Go to 2, 34x; 6. 72°C, 10:00; 7. 4°C	2141 bp band	1 kb < band < 1.5 kb
nsy-1 (ky397)(WRO243) agtcagccatcaagtcctattg		(WRO244) TTTCAACCAACCTGGCC	(WRO268) CGATGATACAAATCACC	Phusion Hot Start II; 1. 98°C, 1:00; 2. 98°C, 0:15; 3. 56.9°C, 0:30; 4. 72°C, 0:20; 5. Go to 2, 34x; 6. 72°C, 10:00; 7. 4°C	atagttcaagtcgattcttcatgcttCaaaaggattcagaacgtagaagatc	atagttcaagtcgattcttcatgcttTaaaaggattcagaacgtagaagatc
nsy-1 (ok593)	(WRO269) agattcatcaatccgagttg	(WRO270) CGAACTCGTTCTTCACGAC	N/A	Phusion Hot Start II; 1. 98°C, 1:00; 2. 98°C, 0:15; 3. 58°C, 0:30; 4. 72°C, 1:45; 5. Go to 2, 34x; 6. 72°C, 10:00; 7. 4°C	~2.5 kb band	~280 bp band
nsy-7 (tm3080)	(WRO237) atgggataaggttggtaactagc	(WRO238) TACAGGTTGCGAAAGGATATTC	N/A	Phusion Hot Start II; 1. 98°C, 1:00; 2. 98°C, 0:15; 3. 65°C, 0:30; 4. 72°C, 1:30; 5. Go to 2, 34x; 6. 72°C, 10:00; 7. 4°C	~700 bp band	~225 bp band
osm-9(ky10)	(WRO235) GATTATATCAAATGGAAGAAGGGAG	(WRO236) GAGTCCTGGAGATTCGGG	(WRO255) AACAAGCGGCAAATGCTAGG	Phusion Hot Start II; 1. 98°C, 1:00; 2. 98°C, 0:15; 3. 58.8°C, 0:30; 4. 72°C, 1:30; 5. Go to 2, 34x; 6. 72°C, 10:00; 7. 4°C	ctttaggcCaatcagccctcc	ctttaggcTaatcagccctcc
ttx-1 (p767)	(WRO191) ccaaatttcaaaa tttgagcactcaaaactctgcct	(WRO193) GTAGATTCCGAATTTGCTAGTGGTAACGTCC	(WRO196) TTCTGGGATTTTTCAGACTTTCC	Phusion Hot Start II; 1. 98°C, 1:00; 2. 98°C, 0:15; 3. 72°C, 0:30; 4. 72°C, 0:40; 5. Go to 2, 34x; 6. 72°C, 10:00; 7. 4°C	atgaacagcggaaattttGtgggttttttaaaattaa	atgaacagcggaaattttAtgggttttttaaaattaa
tax-4 (p678)	(WRO194) CCTACGACGAAAAAATCAGGTGCATACGAC	(WRO195) GGTCCAATGAGATCGTTGAATACTTGTCGAGC	(WRO197) TCAGGTGCATACGACTACG	Phusion Hot Start II; 1. 98°C, 1:00; 2. 98°C, 0:15; 3. 72°C, 0:30; 4. 72°C, 0:40; 5. Go to 2, 34x; 6. 72°C, 10:00; 7. 4°C	gcggccaccggtggtCagccggcatcttccga	gcggccaccggtggtTagccggcatcttccga

*Using Thermo Scientific Phusion Hot Start II High-Fidelity DNA Polymerase.