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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Immunity. 2016 Nov 8;45(5):1038–1051. doi: 10.1016/j.immuni.2016.10.016

Fig 7. Effects of GDF3 on myogenesis.

Fig 7

(A) Increased pSmad2 phosphorylation in regenerating muscles peaking at day 4 post CTX injury. (B-C) Increased Smad2 phosphorylation in primary myoblasts treated with rGDF3. IF images and % of pSMAD2 positive cells are shown. n=3. (D) Heatmap representation of the expression changes of myogenic genes validating the utilized in vitro primary myoblast assay. (E) Heatmap representation of genes that are differentially expressed (min. fold change difference of 1.2X between differentiated myoblasts +/− rGDF3) in the presence of recombinant GDF3 during myoblast differentiation. (F) Heatmap representation of members of the TGF-β superfamily signaling system that are expressed and regulated, or expressed but not regulated in muscle derived macrophages. For non-expressed members, see Fig S7C.