(A) Overlay of monomeric wild-type AIF (gray, PDB: 4BV6) and AIF78
W196A (color) highlights rotation of AIF’s central β-strand and
capping β-hairpin at the FAD/C-domain interface.
(B) The Cβ-clasp region highlighting displacement of the
clasp β-hairpin and the central β-strand.
(C) The H454-S480 hydrogen-bonding network (dashed line) in
monomeric wild-type AIF (left) that is reorganized into S480-H457-Y476 in
dimeric W196A (right). Oxygen, red; nitrogen, blue.
(D) The D444-R450 hydrogen bonding network (dashed line) in
monomeric wild-type AIF (left) that is reorganized to include Q479 in dimeric
W196A (right).
(E) The W477/I445/Y443 border (left) in monomeric wild-type AIF (top
right) aligns to form a contiguous hydrophobic surface against the polar center
of the dimer interface in dimeric W196A (bottom right).