Figure 1.

MyD88-KO mice are easily mobilized by G-CSF and AMD3100. MyD88-deficient and WT mice (as control) were treated with G-CSF (for 6 days at 100 μg/kg per day, subcutaneous injection, upper panel) and AMD3100 (single dose at 5 μg/kg, intraperitoneal injection, lower panel). Experimental mice were killed 6 h after the last G-CSF injection and 1 h after AMD3100 mobilization, and the numbers of WBCs, SKL (Sca-1⁺ c-kit⁺ Lin⁻) cells, HSCs (Sca-1⁺ CD45⁺ Lin⁻) and CFU-GM clonogenic progenitors from PB were evaluated. Results from two separate experiments are pooled together (n=8 mice per group). *P<0.05.