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. 2016 Sep 2;30(12):2416–2419. doi: 10.1038/leu.2016.236

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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The level of HO-1 was evaluated in BMMNCs, and sorted Sca-1⁺ cells collected from WT and MyD88-KO mice were stimulated with G-CSF (140 ng/ml) or AMD3100 (140 ng/ml). Left panel: expression of HO-1 at the mRNA level by real-time PCR. Results from three independent experiments are pooled together and shown as the percentage of expression in WT mice. *P⩽0.05. Right panel: expression of HO-1 by western blotting in BMMNCs and Sca-1⁺ cells purified from WT and MyD88-KO mice. As shown here, the cells from MyD88-KO mice show downregulation of HO-1 compared with WT-derived cells. The experiment was carried out twice with similar results, and a representative blot is shown.