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. Author manuscript; available in PMC: 2017 Dec 6.
Published in final edited form as: Structure. 2016 Nov 10;24(12):2102–2114. doi: 10.1016/j.str.2016.09.017

Figure 6.

Figure 6

Mutations of conserved glycines shift DraNramp’s conformational landscape. (A) Cysteine modification as a function of NEM concentration for five extracellularly-accessible reporters, mapped on a top view of the structure (left). * indicates the metal-binding site. A61C data are repeated from Figures 3C and 4C for comparison. (B) NEM modification for five intracellularly-accessible cysteine reporters as well as T130C, which could not clearly be assigned as intracellular or extracellularly accessible. Reporter positions are indicated on the structure, viewed down the cytoplasmic vestibule. All data are averages ± s.d. (n ≥ 4). See also Figure S5.