(A) Susceptibility of parental H460 cells treated with two doses of
fulvestrant vs. DMSO to lysis by NK cells at various effector-to-target ratios.
(B) Brachyury protein levels and expression of mRNA encoding
for fibronectin (C) and ESR1 (D), relative to GAPDH,
in clonally-derived H1703 cells transfected with pCMV vs. pBr (Clones 1 and 2).
(E) Immunofluorescent analysis of ESR1 (pink signal) protein
expression in H1703 pCMV, pBr-Cl1 and pBr-Cl2 cells (100×
magnification). Green and blue correspond to phalloidin and DAPI staining,
respectively. (F) Susceptibility to TRAIL-mediated lysis in cells
pre-treated with fulvestrant (grey bars) vs. DMSO
(black bars). (G). Susceptibility of H1703
pCMV and H1703 pBr-Cl2 cells treated with fulvestrant (right
panel) vs. DMSO (left panel) to lysis by NK cells
at various effector-to-target ratios. Error bars indicate the standard error of
the mean (SEM) of triplicate measurements. [* p<0.05,
** p<0.01].