Fig. 4.

MiR-1275 inhibits NPC cell growth, and suppresses G1/S transition through down-regulation of HOXB5. a-b. CCK8 assays of CNE-1 and SUNE-1 cells after transfection. c. Shown are representative photos of colony formation assay after transfection for fourteen days. d. Shown are representative photos of BrdU immunofluorescence assay after transfection. Bar = 50 μm. e. Cell-cycle analysis was performed following the treatment CNE-1 and SUNE-1 cells with NC, miR-1275, si-HOXB5 and ASO-1275 for forty eight hours. The DNA content was quantified by flow cytometric analysis. f. Tumor volume in nude mice. g. Tumor weight in nude mice. Each group contained six mice (n = 6); the data are presented as the mean ± SEM; *p < 0.001, compared with the NC group. h. The miR-1275 expression in nude mice. i. The expression of HOXB5 protein in nude mice. Assays were performed in triplicate. *P < 0.05. Means ± SEM was shown. Statistical analysis was conducted using ANOVA