Table 3.
Primers used in this study.
| Gene symbol | Sequence(5′–3′) | Product size (bp) | GenBank no. |
|---|---|---|---|
| RIG-1 F | GCTACCGCCGCTACATCGAG | 224 | EU363349 |
| RIG-1 R | TGCCAGTCCTGTGTAACCTG | ||
| MDA5 F | GCTACAGAAGATAGAAGTGTCA | 120 | KJ451070.1 |
| MDA5 R | CAGGATCAGATCTGGTTCAG | ||
| TLR3 F | GAGTTTCACACAGGATGTTTAC | 200 | JQ910167 |
| TLR3 R | GTGAGATTTGTTCCTTGCAG | ||
| TLR7 F | CCTTTCCCAGAGAGCATTCA | 150 | DQ888645 |
| TLR7 R | TCAAGAAATATCAAGATAATCACATCA | ||
| IL-1β F | TCATCTTCTACCGCCTGGAC | 149 | DQ393268 |
| IL-1β R | GTAGGTGGCGATGTTGACCT | ||
| IL-8 F | AAGTTCATCCACCCTAAATC | 182 | DQ393274 |
| IL-8 R | GCATCAGAATTGAGCTGAGC | ||
| IL-6 F | TTCGACGAGGAGAAATGCTT | 150 | AB191038 |
| IL-6 R | CCTTATCGTCGTTGCCAGAT | ||
| IL-10 F | CTGACCTCCTACCAGCGAAG | 179 | NM001310368 |
| IL-10 R | CTCCATGTAGAACCGCATCA | ||
| IFN-α F | TCCTCCAACACCTCTTCGAC | 232 | EF053034 |
| IFN-α R | GGGCTGTAGGTGTGGTTCTG | ||
| IFN-β F | AGATGGCTCCCAGCTCTACA | 210 | KM035791.1 |
| IFN-β R | AGTGGTTGAGCTGGTTGAGG | ||
| IFN-γ F | GCTGATGGCAATCCTGTTTT | 247 | AJ012254 |
| IFN-γ R | GGATTTTCAAGCCAGTCAGC | ||
| MX F | TGCTGTCCTTCATGACTTCG | 153 | GU202170.1 |
| MX R | GCTTTGCTGAGCCGATTAAC | ||
| OAS F | TCTTCCTCAGCTGCTTCTCC | 187 | KJ126991.1 |
| OAS R | ACTTCGATGGACTCGCTGTT | ||
| PKR F | AATTCCTTGCCTTTTCATTCAA | 109 | Unpublished |
| PKR R | TTTGTTTTGTGCCATATCTTGG | ||
| GAPDH F | ATGTTCGTGATGGGTGTGAA | 176 | AY436595 |
| GAPDH R | CTGTCTTCGTGTGTGGCTGT |