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Excitation (dash lines) and emission (solid lines) spectra of 1.0X EvaGreen (green lines), SYBR Green (purple lines), PicoGreen (red lines), AccuClear (aqua lines), AccuBlue NextGen (orange lines) and 100 nM YOYO-1 (blue lines) free dye. Spectra were recorded with a LS55 fluorescence spectrometer (Perkin Elmer) (excitation slit: 2.5 nm, emission slit: 3.5 nm).
