Figure 6.

Erianin inhibits OS xenograft growth in vivo. 143B cells were orthotopically inoculated into the left tibia of BALB/c-nu mice. One week after tumor inoculation, mice were randomly divided into two groups for treatment. Intraperitoneal administration of vehicle or erianin (2 mg/kg) every other day for seven times. (a) Tumor volume was measured every week. (b) Erianin treatment resulted in significantly lower than control group. (c) Body weights were measured every week. (d) Detection of apoptosis in tumor tissues by TUNEL assay. The expression of PCNA (proliferating cell nuclear antigen), cleaved caspase-3, and p-JNK were examined by immunohistochemistry. (e) No major organ-related toxicities was observed. H&E staining was used to evaluate the histology. Data represent mean±S.D. *P<0.05, significantly different compared with control