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. 2016 Jun 2;7(6):e2248. doi: 10.1038/cddis.2016.145

Figure 3.

Figure 3

RNCR3 knockdown aggravates hypercholesterolemia and increases inflammatory factor releases. Four-week-old male ApoE−/− mice were fed high-fat diet containing 0.15 cholesterol and 20% fat for 16 weeks. They were received a subcutaneous injection of scrambled shRNA (Scr) or RNCR3 shRNA viral vector (R), or PBS. shRNA injection was started at 4 weeks after feeding high-fat diet. Viral vector was injected once every 2 weeks. (a, b) Plasma cholesterol and triglycerides levels were detected in PBS-, scrambled shRNA-, and RNCR3 shRNA-injected mice (n=6 per group). (c) Fast protein liquid chromatographic (FPLC) lipoprotein profiles from the pooled plasma (n=6 per group) of PBS-, scrambled shRNA-, and RNCR3 shRNA-injected mice. (d–f) Plasma levels of TNF-α, CCL2, and IL-6 protein from PBS-, scrambled shRNA-, and RNCR3 shRNA-injected mice were quantified by ELISAs (n=6 per group). *P<0.05 versus PBS-injected group. HDL, high-density lipoprotein; LDL, low-density lipoprotein; VLDL, very low-density lipoprotein