Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 2;7(6):e2248. doi: 10.1038/cddis.2016.145

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

RNCR3 regulates endothelial cell function by acting as a ceRNA. (a) HUVECs were transfected with different miRNA mimics, or left untreated (Ctrl) for 48 h. qRT-PCRs were conducted to detect RNCR3 levels. The data was expressed as relative change compared with Ctrl group. *P<0.05 versus Ctrl group. (b) HUVECs were transfected with Ago2 siRNA, scrambled siRNA, or left untreated (Ctrl). miR-185-5p or RNCR3 levels were detected using qRT-PCRs. *P<0.05 versus Ctrl group. (c) KLF2 was predicted as a target gene of miR-185-5p using TargetScan. The position of miR-185-5p binding site on KLF2 was shown. (d) KLF2 (RLuc-KLF2-WT) or mutant (RLuc-KLF2-Mut) was co-transfected with miR-185-5p mimic, scrambled miRNA mimic, or left untreated. Luciferase activity was detected using the dual luciferase assay. *P<0.05 versus untransfected group. (e–h) HUVECs were treated as shown. Cell viability was detected using MTT assay (e, g). A representative image for Ki67 staining along with quantification analysis data was shown. Scale bar, 20 μm (f, h). *P<0.05 versus Ctrl group. ^#Significant difference between the marked groups (^#P<0.05)