Figure 2.

Downregulation of cdr2 in MPP⁺-injected rat brains. (a) Rats received a unilateral stereotaxic injection of saline (sham) or MPP⁺ (100 μmol) into the striatum (STR). The indicated periods (W, week) after surgery, and cdr2 levels in the ipsilateral (Ipsi) or contralateral (Cont) SN were determined by immunoblot analysis using anti-cdr2 antibody. Mouse monoclonal anti-TH antibody was used to assess the severity of damage to dopaminergic neurons. (b) At 2 weeks after stereotaxic injection of MPP⁺ into the STR, SN sections were immunolabeled with anti-TH antibody and human PCD patient CSF followed by incubation with fluorescence-tagged secondary antibodies. Arrows indicate cdr2 staining in TH-positive neurons after MPP⁺ treatment. Scale bar: 40 μm. The mean intensity of TH and cdr2 fluorescence in the Ipsi side was measured and expressed as a percentage of that in the sham-treated Cont side. Data were obtained from five randomly selected microscopic fields per section, with 16–20 sections per rat (~8–10 neurons per section). Data are shown as the mean±S.D. of 4–5 rats. *P<0.05; **P<0.01; n.s., not significant