Figure 3.

KLF4 is a potential tumor suppressor in human gastric cancer. (a) Statistical analysis of KLF4 expression in normal and gastric cancer from TCGA database. (b) Statistical analysis of correlation between expression level and methylation of KLF4 in gastric cancer patients. (c) Western blot of KLF4 and target genes in AGS cells infected with control or KLF4-carrying adenovirus. (d) Growth curves of AGS cells infected with control or KLF4-carrying adenovirus. Data are represented as mean±S.D. (*P<0.005). (e) Real-time PCR of MUC2 and KLF4 mRNA expression in AGS cells infected with control or KLF4-carrying adenovirus. Data are represented as mean±S.D. (f) Representative image of antral glands isolated from adult mice. (g) RT-PCR analysis of gene expression in Klf4-deleted antral glands. Three control mice and three Rosa-Cre⁺;Klf4 ^fl/fl mice were used in this experiment