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. 2016 Jun 9;7(6):e2257. doi: 10.1038/cddis.2016.161

Figure 3.

Figure 3

DJ-1 disruption has no effect on LPC proliferation in vitro. (a) Immunofluorescence of CK19/BrdU in LPCs isolated from WT and DJ-1 KO mice after DDC feeding 4 weeks (× 200). (b) The proportions of BrdU+ cells in total CK19+ cells were quantified. (c and d) Relative DJ-1 mRNA and protein level were analyzed in non-targeted cells (NT) and two established DJ-1 KD cell lines (KD1 and KD2). GAPDH (glyceraldehyde 3-phosphate dehydrogenase) was used as a loading control. (e) Representative IHC of BrdU staining in NT, KD1 and KD2 cell lines ( × 200). (f) BrdU+ cells in NT, KD1 and KD2 cell lines were quantified. *P<0.05. Data are presented as means±S.E.M.