Figure 1.

HSC differentiation in Mysm1^−/− mice. (a) BM cells from 8- to 10-week-old WT or Mysm1^−/− mice were analyzed by FACS for the frequency of LSK; (b) for the frequencies of LSK IL-7R⁺ and LSK IL-7R⁻; and (c) for the death propensity of LSK by 7AAD staining. The data shown are from one of three independent experiments (n=4). (d) Eight- to ten-week-old WT or Mysm1^−/− mice received 1 mg of BrdU i.p. injection daily for 5 days. Incorporation of BrdU was analyzed by FACS to determine the proliferation of LSKs (n=4). (e) BM cells from 8- to 10-week-old WT or Mysm1^−/− mice were analyzed by Hoechst 33258/Pyronin Y staining for cell-cycle phases of LSKs (n=4). (f) Eight- to ten-week-old WT or Mysm1^−/− mice were injected with 2 mg of BrdU. One hour later, the mice were euthanized and BM cells were analyzed for cell-cycle kinetics of LSKs (n=4). (g) Five- to six-week-old WT or Mysm1^−/− mice received i.p. injection of 5-FU at 0, 30, or 60 μg/g of body weight. Forty-eight hours post injection, BM cells were harvested from the euthanized mice and analyzed for frequencies (top) and cell death (middle) of LSKs, and for expression of IL-7R (lower) in Lin⁻ BM cells. Data are representative of three independent experiments. *P<0.01; **P<0.05