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. 2016 Jun 9;7(6):e2260. doi: 10.1038/cddis.2016.162

Figure 6.

Figure 6

IRF2 and/or IRF8 suppresses p53 transcription in Mysm1−/− HSCs. (a) BM cells from ~8-week-old WT or Mysm1−/− mice (n=4) were analyzed by FACS for p53 protein levels in Lin− BM cells or LSKs. (b and c) ~8-week-old normal mice were injected with Poly(I:C) (20 μg/g body weight). Two days post injection, BM cells from Poly(I:C)-treated or non-treated mice (n=4) were analyzed by FACS for the protein levels of Mysm1 (b) or p53 (c) in Lin− BM cells or LSKs. (d) Lin− BM cells from ~8-week-old WT or Mysm1−/− mice (n=4) were analyzed by qPCR for expression of p53 mRNA after in vitro stimulation with 100 ng of Poly(I:C) for 4 h. (e) Lin− BM cells from ~8-week-old Mysm1−/− mice (n=4) were transduced with the indicated RV vectors. Two days later, the transduced Mysm1−/− Lin− cells were sorted and the GFP+ cells were analyzed for expression of p53 mRNA by qPCR. Bar graphs show means of three experiments±S.D. *P<0.05