Figure 3.
Inhibition of β-catenin pathway induced by transfection of an anti-TCF4-specific siRNA in CaCo2 and HCT116 cell lines. (a) siRNA-mediated silencing of TCF4 results in the induction of MUCDHL, CDX2, and p21waf1 transcripts. Cells were transfected with a scrambled siRNA (Cont. siRNA) or with a mix of siRNA against TCF4 (TCF4 siRNA) and analyzed after 72 h by QRT-PCR. Data are presented as in Figure 2, panel b. Results are represented as mean±S.E.M. values and asterisks indicate statistically significant results (P<0.05). (b) Western blot analysis confirming the results of the QRT-PCR reactions. This analysis was performed on cell samples under the same experimental conditions and the results presented as explained in Figure 2, panel b