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. 2016 Jun 16;7(6):e2263. doi: 10.1038/cddis.2016.163

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Deprivation of WNT3a in culture medium of human colonic organoids induce the mRNA expression of MUCDHL and CDX2. (a) Colonic organoids were cultured in the presence of gradually reduced concentrations of WNT3a (1/5 and 1/10 of the standard concentration) or under differentiation-inducing conditions characterized by the complete withdrawal of all β-catenin agonists from culture medium. QRT-PCR analysis was performed after 72 h and the mean values obtained by four independent experiments presented as in previous figures. Results are represented as mean±S.E.M. values and asterisks indicate statistically significant results (P<0.05). (b) Representative bright-field image of colonic organoids grown under standard (Cont.) or differentiation conditions. (c) Analysis of circularity performed on colonic organoids cultured under standard (Cont.) or differentiation conditions. The dot plot shows the results of two independent experiments represented as mean±S.E.M. (***P<0.0001)