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. 2016 Jun 16;7(6):e2263. doi: 10.1038/cddis.2016.163

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Transfection of CaCo2 cells with an anti-CDX2 siRNA interferes with upregulation of μ-protocadherin expression induced by stimulation with 5-ASA. (a) CaCo2 cells were transfected with a scrambled siRNA (Cont. siRNA) or a mix of anti-CDX2 siRNAs (CDX2 siRNA) and stimulated with 5-ASA to induce μ-protocadherin expression. The effect determined on the expression of CDX2 and MUCDHL mRNAs was then analyzed by QRT-PCR. Results are represented as mean±S.E.M. values and asterisks indicate statistically significant results (P<0.05). (b) The effect determined on the expression of the same genes at the protein level was evaluated by western blot analysis. Data were presented according to the same modalities of the previous figures