Figure 7.

ER stress was involved in neutrophil apoptosis in vitro. (a) BiP, ATF4 and CHOP protein levels in mouse neutrophils stimulated with Ang II for the indicated times. (b) Bar graph shows the quantifications of BiP, ATF4 and CHOP relative to GAPDH (n=4 in each group). (c) The apoptosis of WT and CHOP KO mouse neutrophils was analyzed by annexin V and PI staining stimulated with Ang II (1 μmol/l) for 24 h. (d) Neutrophil apoptosis was calculated as the percentage of annexin V⁺ cells (both annexin V⁺PI⁻ and annexin V⁺PI⁺, n=3 in each group) to total cells. (e) Western blot analysis of Bcl-XL and Bcl-2 in WT and CHOP KO mouse neutrophils stimulated with Ang II (1 μmol/l) for 24 h in vitro. (e) Bar graph shows the quantifications of Bcl-XL and Bcl-2 in relative to GAPDH (n=4 in each group). *P<0.05 compared with time zero. **P<0.01 compared with time zero. ^#P<0.05 compared with the WT group