Fig. 1.

616452 induces megakaryopoiesis in human BM CD34⁺ cells. A representative plot of the effectiveness of the chemical 616452, and how the induction of megakaryopoiesis was induced (a). BM CD34⁺ cells were induced with 616452 (10 uM) for 8 days and compared to control (b, c). The induced cells were stained by live cell staining for CD41 (d) and analyzed by flow cytometry on day 5 (e). Cells were also collected for Giemsa-Wright staining after 10 days of culture (f), to illustrate the multi-nucleated and lobular nature of the nuclei