Fig. 6.

Gene expression profile and RT-PCR of cells after 4 days of induction. Gene expression profile of CB CD34⁺ cells induced for 2 and 4 days compared to control (a). There are noticeably more genes expressed and at higher levels on 4 days of induction compared to that of day 2. There are 18 genes total upregulated on day 2, and by day 4, there were 38. On the right is a heat map illustrating the values of expression in terms of fold increase over control. Green is indicative of upregulation while red is downregulation. Quantification of the expression level of related genes in human umbilical cord blood cells treated with or without 616452 harvested at day 4 by quantitative PCR (b). 616452 untreated umbilical cord blood cells were used as controls as shown in the figure. The values were normalized by GAPDH expressed as relative quantitation (RQ), the error bars depict the SD (n = 3)