Figure 8.

Expression of wild-type pfh1⁺ abolishes the ability of pfh1-R20 or pfh1-R23 to suppress the temperature-sensitive growth defect of cdc24-M38. The cdc24-M38 pfh1-R20 (M38 R20) or the cdc24-M38 pfh1-R23 (M38 R23) double mutant cells (strains 24R20 and 24R23, respectively) were transformed with pREP81x (vector) alone or pREP81x-pfh1 expressing wild-type pfh1⁺ (Pfh1) as denoted at the left-hand side of figure. Each transformant was grown in liquid media and the cells obtained were spotted in 10-fold dilutions (10⁵, 10⁴, 10³, 10² and 10¹ cells) onto EMM plates or EMM plates containing thiamine (5 μg/ml). The plates were incubated for 3 days at 30°C and at 37°C or 7 days at 18°C as shown.